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  • D1445 - Anti-DPP4 (61-75) antibody produced in rabbit

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D1445 Sigma-Aldrich

Anti-DPP4 (61-75) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ADABP, Anti-ADCP2, Anti-CD26, Anti-DPPIV, Anti-Dipeptidyl peptidase 4, Anti-TP103

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, DM-DP, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:2,000
species reactivity   human
mol wt   antigen ~88 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P27487
Gene Information   human ... DPP4(1803)

Description

Immunogen

synthetic peptide corresponding to amino acids 61-75 of human DPP4

Application

Anti-DPP4 antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

DPP4 is a cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. It regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. It removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini with proline as the penultimate residue. It is ubiquitously expressed in epithelial and endothelial cells and plays a role in the migration and invasion of human endothelial cells in collagenous matrices. ADA-DPP4 interaction on the cell surface regulates lymphocyte-epithelial cell adhesion. Dipeptidyl peptidase activity of this protein is inhibited by the binding of Simpson-Golabi-Behmel syndrome causative glypican-3.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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