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D1556 Sigma-Aldrich

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym: Iodixanol solution



Related Categories Cell Biology, Density Gradient Media and Cell Separation, General Stem Cell Biology, Gradients, Hematology and Histology,
Quality Level   200
application(s)   hematology: suitable
  histology: suitable
SMILES string   CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I
InChI   1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)



OptiPrep Density Gradient Medium has been used in the non enveloped virion isolation from bronchoalveolar lavage.
Density gradient is also suitable for:
• isolation of cells and cell organelles
• virus purification
• isolation of lipoproteins and macromolecules


250 mL in poly bottle

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Alere Technologies AS

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?
Percoll and Histopaque must be removed prior to use through washing.If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.
What is the appearance of Product D1556, OptiPrep?
The solution may be clear to slightly yellow.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I store solutions of Product D1556, OptiPrep Density Gradient Medium?
After diluting Product D1556, OptiPrep Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.
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