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D1556 Sigma

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym: Iodixanol solution

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Density gradient suitable for isolation of cells and cell organelles, virus purification, and isolation of lipoproteins and macromolecules.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Packaging

250 mL in poly bottle

Legal Information

OptiPrep is a trademark of Alere Technologies AS

Price and Availability


LC-MS Grade Solvents and Reagents

Tissue Diagnostics

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?
Percoll and Histopaque must be removed prior to use through washing.  If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.  In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.
What is the appearance of Product D1556, OptiPrep?
The solution may be clear to slightly yellow.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?
After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

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