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D1816 Sigma-Aldrich

RED KlenTaq® DV ReadyMix

Synonym: KlenTaq® DV ReadyMix

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Properties

concentration   4 units/reaction (50 μl reaction volume)
shipped in   wet ice
storage temp.   −20°C

Description

Application

KlenTaq® DV ReadyMix is an excellent blend for applications requiring high PCR yields from less complex, thoroughly purified templates.

Features and Benefits

• Better efficiency and yield than Taq-based formulations, due to the higher processivity of KlenTaq-1
• Magnesium optimization is unnecessary, since KlenTaq works over a broad magnesium range
• Excellent amplification up to 3 kb for less complex templates (e.g., plasmid and lambda DNA) and 1.5 kb on human genomic DNA
• When performing large numbers of PCR reactions, KlenTaq DV ReadyMixes can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent performance
• RED KlenTaq DV ReadyMix provides immediate confirmation that not only has the enzyme been added, but that proper mixing has occurred. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis

Other Notes

KlenTaq® DV ReadyMixes combine the performance benefits of KlenTaq DNA polymerase with the convenience of a ReadyMix. This ready-to-use mixture of KlenTaq-1 DNA polymerase (a 5′-exo-minus, N-terminal deletion of Taq DNA polymerase), 99% pure deoxynucleotides, reaction buffer and a small amount of a proofreading DNA polymerase is provided in a 2× concentrate. RED KlenTaq DV ReadyMix includes Sigma′s proprietary inert red dye.
For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Reaction volumes can be scaled down, if desired.

Principle

KlenTaq-1 is more efficient and more processive than either native Taq DNA polymerase or other N-terminal deletions of Taq. This means a higher amount of product is generated with the same number of cycles than when using Taq DNA polymerase.
In RED KlenTaq DV ReadyMix, the inert red dye allows for easy confirmation of enzyme addition and visualization of complete mixing during setup. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. There is no need to add a loading buffer/tracking dye prior to electrophoresis. The red dye migrates at approximately the same rate as a 125 base pair fragment.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
KlenTaq is a registered trademark of Wayne Barnes.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

KlenTaq is a registered trademark of Wayne Barnes

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

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