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D1878 Sigma-Aldrich

2,6-Dichloroindophenol sodium salt hydrate

suitable for vitamin C determination, BioReagent

Synonym: 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, 2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, DCIP, DPIP

  • CAS Number 1266615-56-8

  • Empirical Formula (Hill Notation) C12H6Cl2NNaO2 · xH2O

  • Molecular Weight 290.08 (anhydrous basis)

  •  Beilstein/REAXYS Number 3641229

  •  EC Number 210-640-4

  •  MDL number MFCD00150014

  •  PubChem Substance ID 57654075

  •  NACRES NA.47



Related Categories Analytical Reagents, Analytical/Chromatography, Application Specific Reagents, Cell Biology, Core Bioreagents,
Quality Level   200
product line   BioReagent
form   powder
application(s)   hematology: suitable
  histology: suitable
solubility   H2O: soluble 10 mg/mL, clear, blue to very deep blue
suitability   suitable for vitamin C determination
Featured Industry   Diagnostic Assay Manufacturing
Food and Beverages
storage temp.   room temp
SMILES string   O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2
InChI   1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1


General description

2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.


2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
• As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
• To compose UVB specific dosimeter.
• Quantification of vitamin C (L-ascorbic acid) in fruit juices.
• As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.
• Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water.Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.
HasProduct D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid isred and blue in alkali. The product is colorless when reduced.
Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.
Are there substances known to interfere in Vitamin C determination?
Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.
Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.
Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?
Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) andK. Hiromi et al., Anal. Biochem. 101, 421, (1980).
Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
No. Unfortunately, we have not performed solution stability studies on this product.
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