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D1878 Sigma

2,6-Dichloroindophenol sodium salt hydrate

suitable for vitamin C determination, BioReagent

Synonym: 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, 2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, DCIP, DPIP

  • CAS Number 1266615-56-8

  • Empirical Formula (Hill Notation) C12H6Cl2NNaO2 · xH2O

  • Molecular Weight 290.08 (anhydrous basis)

  •  Beilstein Registry Number 3641229

  •  EC Number 210-640-4

  •  MDL number MFCD00150014

  •  PubChem Substance ID 57654075

  • Popular Documents:  FTIR (PDF)  



Related Categories Analytical Reagents, Analytical/Chromatography, Application Specific Reagents, Cell Biology, Core Bioreagents,
product line   BioReagent
form   powder
solubility   H2O: soluble 10 mg/mL, clear, blue to very deep blue
suitability   suitable for vitamin C determination



2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
• As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
• To compose UVB specific dosimeter.
• Quantification of vitamin C (L-ascorbic acid) in fruit juices.
• As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.
• Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.

General description

2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.

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Safety Information

NONH for all modes of transport
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Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water.  Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.
Has Product D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid is  red and blue in alkali. The product is colorless when reduced.
Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.
Are there substances known to interfere in Vitamin C determination?
Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.
 Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.
Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?
Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) and  K. Hiromi et al., Anal. Biochem. 101, 421, (1980).
Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?
No. Unfortunately, we have not performed solution stability studies on this product.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


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