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D2178 Sigma-Aldrich

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

clone DAPK-55, ascites fluid

Synonym: Anti-DAP Kinase

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Other Apoptosis-related Proteins,
conjugate   unconjugated
clone   DAPK-55, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: 1:250 using COS-7 cell extract
species reactivity   monkey, human
mol wt   antigen mol wt 160 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P51397
Gene Information   human ... DAP(1611)

Description

General description

The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain.

Immunogen

recombinant human DAP kinase expressed in bacteria.

Application

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse is suitable for immunoprecipitation, microarray and western blotting at a working dilution of 1:250 using COS-7 cell extract. It may be used for the detection and quantification of kinase activity of DAP Kinase using various immunochemical assays such as immunoblot and immunoprecipitation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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D4941 Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal, clone DKPS308, purified from hybridoma cell culture
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