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D2189 Sigma-Aldrich

[Glu52]--Diphtheria toxin from Pseudomonas fluorescens expressed recombinant CRM197

lyophilized powder

Synonym: CRM 197, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae

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Description

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing sodium phosphate, sucrose and polysorbate 80

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?
The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.
What kind of mutant, isProduct D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?
Product No. D0564 is the wild-type toxin and possesses enzymatic activity.The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A.,J. Biol. Chem., 248, 3851-3854 (1973).
What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?
On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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