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  • D219 - Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse

D219 Sigma

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Calcium Channels, Antibodies to Ion Channels,
species reactivity   guinea pig, mouse, rabbit, rat
application(s)   immunohistochemistry (frozen sections): 1:500
  western blot: 1:500
clone   20A, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 143 kDa (reduced)
  antigen mol wt 220 kDa (non-reduced)
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)
biological source   mouse
conjugate   unconjugated



Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.


Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

Other Notes

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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