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D2444 Sigma-Aldrich

Anti-β-Defensin 3 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DEFB103A, Anti-HBD3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood, Antibodies to Proteins of Inflammation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: 1:2
  western blot: 1:500-1:1,000
species reactivity   human
mol wt   antigen mol wt ~5 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
Gene Information   human ... DEFB103A(55894)
mouse ... Defb3(27358)
rat ... Defb3(641623)

Description

Immunogen

synthetic peptide corresponding to an internal sequence of human β-Defensin 3.

Application

Anti-β-Defensin 3 antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:500 to 1:1000 and for immunohistochemistry at 1:2 dilution. It was used for a quantitative immunohistochemical study of HBD-3 on skin lesions from patients having Kaposi’s sarcoma in a study. It was used as a primary antibody for western blot analysis of oral mucosa and extraoral skin samples to study the quantitative comparison of the expression of antimicrobial peptides.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Target description

β-Defensin 3 encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

hBD-3 m-RNA expression is induced by tumor necrosis factor a and contact with bacteria. Therefore, it might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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D9565 β-Defensin 1 human, ≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder (from 10 mM acetic acid)
D9690 β-Defensin 2 human, recombinant, expressed in E. coli, lyophilized powder (from 10 mM acetic acid)

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