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D2886 Sigma-Aldrich

DNA Ligase from T4-infected Escherichia coli

buffered aqueous glycerol solution

Synonym: Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, T4 DNA Ligase



Related Categories 6.5.x.x Phosphoric ester, 6.x.x.x Ligases, Application Index, Biochemicals and Reagents, Core Bioreagents,
Quality Level   200
grade   for molecular biology
form   buffered aqueous glycerol solution
activity   4,000 U/mL
mol wt   68 kDa
storage temp.   −20°C
Gene Information   bacteriophage T4 ... 30(1258680)



Suitable for:
• Ligation of blunt ended or cohesive DNA fragments
• Ligation of cloning vector and restriction insert fragments
• Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids
• Couple RNA single strands by bridging oligonucleotide adapters

Biochem/physiol Actions

T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.


T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.

Unit Definition

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.

Other Notes

T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


T4 DNA Ligase

T4 DNA Ligase (Product No. D2886) catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt ...
Keywords: Cloning, Peptide synthesis, Polymerase chain reaction, Polymorphisms

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