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D2902 Sigma-Aldrich

Dulbecco’s Modified Eagle’s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym: DME, DMEM

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
form   powder
application(s)   cell culture | mammalian: suitable
solubility   H2O: soluble 10 g/L, clear
components   HEPES: no
sodium pyruvate: yes
glucose: low
phenol red: no
L-glutamine: yes
NaHCO3: no
shipped in   ambient
storage temp.   2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium - low glucose is used in cell line culture (used in medium for the growth of cells) and for their maintenance. It is also used for tissue culture.

Quantity

Formulated to contain 10.0 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

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Peer-Reviewed Papers
15

References

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