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D2906 Sigma-Aldrich

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym: DME/F-12, 1:1 mixture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
Quality Level   GMP
form   powder
suitability   suitable for cell culture
components   phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
NaHCO3: no
L-glutamine: yes
HEPES: 15 mM
storage temp.   2-8°C

Description

General description

Suitable for growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Single- & multi-channel pipettes from BRAND

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Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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