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D3514 Sigma

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate

≥80% (elemental analysis), powder

Synonym: DIDS, Disodium 4,4′-diisothiocyanatostilbene-2,2′-disulfonate



Related Categories Amine to Amine, Biochemicals and Reagents, Chemical Biology, Chemical Synthesis, Crosslinkers,
assay   ≥80% (elemental analysis)
form   powder
color   yellow
solubility   0.1 M potassium bicarbonate: 50 mg/mL
storage temp.   2-8°C



A negatively charged homobifunctional cross-linking reagent. The isothiocyanate groups react with primary amines at pH 9.0-10.0.

Biochem/physiol Actions

A specific inhibitor of cellular anion permeability used in cell transport studies.


1 g in poly bottle

100, 250 mg in poly bottle

25 mg in glass insert

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Safety Information

Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the solubility of 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt (Product No. D3514)?
Product No. D3514 is soluble in 0.1 M KHCO3 (50 mg/mL) yielding a hazy, yellow-green solution. This may require heat for complete solubilization. DIDS is also soluble in DMSO.
What is the solution stability of 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt (Product No. D3514)?
We have no specific solution stability data for Product No. D3514, however, isothiocyanates are unstable in water and should not be stored in aqueous solutions.
What are the fluorescent properties of 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt (Product No. D3514)?
Product No. D3514 has an excitation maximum at a wavelength of 342 nm, and an emission maximum at a wavelength of 418 nm, measured in water.
What are the spectral properties of 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt (Product No. D3514)?
The spectral properties of Product No. D3514 are: λmax: 220 nm, 265 nm Extinction coefficient: EmM = 3000 (280 nm, water), EmM = 54 (342 nm, water).  This information is from J. Membrane Biol., 29, 147-177 (1976).  
Is there a reference for the use of 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt (Product No. D3514) as a cross-linking reagent?
Product No. D3514 binds covalently (irreversibly) to the outer surface of human erythrocyte membrane protein, functioning as an anion transport inhibitor. It also can be used to cross-link membrane anion transport sites.  A good cross-linking reference is J. Membrane Biol., 29, 147-177 (1976).
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