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  • D4551 - N,N-Dimethylformamide

D4551 Sigma


for molecular biology, ≥99%

Synonym: DMF, NSC 5356

  • CAS Number 68-12-2

  • Linear Formula HCON(CH3)2

  • Molecular Weight 73.09

  •  Beilstein Registry Number 605365

  •  EC Number 200-679-5

  •  MDL number MFCD00003284

  •  PubChem Substance ID 24893883



Related Categories Biochemicals, Core Bioreagents, Dimethylformamide (DMF), Life Science Reagents for Protein Expression and Purification, Life Science Reagents for Transfection,
grade   for molecular biology
vapor density   2.5 (vs air)
vapor pressure   2.7 mmHg ( 20 °C)
assay   ≥99%
form   liquid
autoignition temp.   833 °F
expl. lim.   15.2 %
impurities   ≤0.5% water
refractive index   n20/D 1.430(lit.)
bp   153 °C(lit.)
mp   −61 °C (lit.)
density   0.944 g/mL(lit.)
suitability   suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.
storage temp.   room temp



Solvent for many hydrophobic organic compounds.

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.


250, 500 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 2265 3 / PGIII
WGK Germany 
Flash Point(F) 
136.4 °F
Flash Point(C) 
58 °C
Protocols & Articles


N,N-Dimethylformamide Molecular Biology Reagent

DMF is a polar organic solvent that is widely used in the synthesis of organic compounds. The high solvent power and stability of DMF at elevated temperatures contribute to its utility. DMF is a solv...
Keywords: Affinity chromatography, Capillary electrophoresis, Carboxylations, Chromatography, Crystallization, Electrophoresis, High performance liquid chromatography, Melting, Molecular biology, Separation, Solvents

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation


Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Related Content

Pressure-Temperature Nomograph Interactive Tool

The Pressure-Temperature Nomograph tool is an application of the Clausius-Clapeyron Equation, which assumes the heat of vaporization is a constant over a pressure range. Antoine´s Equation gets aroun...
Keywords: Evaporation, Vaporization

Properties of Solvents Table

a. Gant, R., LC/GC 10 (7), 515 (1992) 1. At 20 °C 2. At 20 °C +/- 5 °C relative to water at 4 °C 3. At 10 mmHg pressure 4. At l=589.32 nm
Keywords: Gas chromatography, High performance liquid chromatography

Peer-Reviewed Papers


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