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D4567 Sigma-Aldrich

Anti-DNMT1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-DNA methyltransferase

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:100-1:200 using mouse COS-7 cells
  microarray: suitable
  western blot: 1:1,000-1:2,000 using nuclear extracts of human HEK 293T cells
species reactivity   human, rat, mouse
mol wt   antigen ~180 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P26358
Gene Information   human ... DNMT1(1786)
mouse ... Dnmt1(13433)

Description

General description

In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.

Immunogen

synthetic peptide corresponding to amino acids 1581-1595 of rat DNMT1, conjugated to KLH via an N-terminal added lysine residue. The sequence is conserved in human and mouse.

Application

Anti-DNMT1 antibody produced in rabbit is suitable for microarray, immunofluorescence at a working dilution of 1:100-1:200 using mouse COS-7 cells and western blot analysis at 1:1000-1:2000 dilution using nuclear extracts of human HEK 293T cells. Antibodies reacting specifically with DNMT1 may be useful for the study of chromatin remodeling effects on gene expression. It was used as a primary antibody at 1:2000 working dilution to detect DNMT1 protein in protein sample extracted from colon cancer cells transfected with DNMT1 vectors.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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