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D4654 Sigma-Aldrich

M13mp9 Phage DNA Double stranded, (RF-I) from Escherichia coli JM101

buffered aqueous solution

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Properties

form   buffered aqueous solution
mol wt   4.8 MDa (7,229 bp)
shipped in   dry ice
storage temp.   −20°C

Description

Application

M13 is a single stranded DNA bacteriophage that infects male Escherichia coli strains. A chronic infection is set up during which the intracellular replicative form of the phage (RF-I, double stranded DNA) serves as template for single stranded ([+] strand) DNA production. The single stranded DNA, packaged in phage coat proteins, is extruded through the host cell wall into the medium in large quantities.
M13mp8/9 and M13mp18/19 are M13 derivatives. Each contains a multiple cloning site (MCS) within a portion of the gene for β-galactosidase. This DNA segment corrects a defect in β-galactosidase production by the host strain. The MCS regions of M13mp8/9 and M13mp18/19 contain 9 and 13 unique restriction endonuclease sites, respectively.
Foreign DNA incorporated at the multiple cloning site results in loss of β-galactosidase production and consequently the inability to use lactose as a carbon source. Such lactose negative strains are detected as white plaques on medium containing X-Gal, a chromogenic substrate for β-galactosidase.
The intracellular, double stranded (RF-I) form of the M13 phage can be manipulated like plasmid DNA, while the extracellular, single stranded DNA is an excellent source of material for determining the sequence of insert DNA by the dideoxy procedure. The multiple cloning site of M13mp9/19 is in the opposite orientation relative to the cloning site of M13mp8/18.

Features and Benefits

• DNA cloning vectors.
• Single stranded DNA serves as a sequencing template.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

Analysis Note

Unique sites: Within the β-galactosidase gene - Bgl I, Mst II, Pvu I; other unique sites - Aha II, ApaL I, Ava II, Bal I, Ban II, Bgl II, Bsm I, Dra III, HgiD I, Nae I, Nar I, Sau I, Sin I, SnaB I, Sno I.
Note: Attempts to clone foreign DNA at these "other" sites will likely lead to inactivation of essential phage functions.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000

Documents

Certificate of Analysis

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Protocols & Articles
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