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  • D4692 - Anti-DNMT1 antibody produced in rabbit

D4692 Sigma-Aldrich

Anti-DNMT1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DNA methyltransferase



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 0.5-1.0 μg/mL using nuclear extracts of 293T or HeLa cells
species reactivity   human, mouse
mol wt   antigen ~180 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   0.8-1.2 mg/mL
UniProt accession no.   P26358
Gene Information   human ... DNMT1(1786)


General description

In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.


synthetic peptide corresponding to amino acids 72-78 of human DNMT1, conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in mouse and is different from the rat sequence by one amino acid.


Anti-DNMT1 antibody produced in rabbit is suitable for microarray and immunoblotting at a working concentration of 0.5-1.0μg/mL using nuclear extracts of 293T or HeLa cells. It was used at 1:1000 working dilution for immunoblot analysis in a study to analyze whether some miRNAs are aberrantly expressed and target DNMT1 in NiS (nickel sulfide)-transformed cells. It was used for immunoprecipitation of DNMT1 from nuclear lysates of colon cancer cells transfected with DNMT1 vectors in a study. It was used as a primary antibody at a working dilution of 1:1000 for western blot analysis of total protein extracted from left murine testis tissue exposed to low-dose-rate radiation in a study.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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