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  • D4941 - Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal

D4941 Sigma-Aldrich

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal

clone DKPS308, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   DKPS308, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using 293T (human embryonal kidney) cells transfected with DAP-kinase expression vector.
species reactivity   human
mol wt   antigen ~160 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P53355
Gene Information   human ... DAPK1(1612)


General description

Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active.


Phosphopeptide corresponding to amino acids 303-312 (pSer308) of human DAP-kinase, conjugated to KLH.


Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in:
• western blot assay
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used in indirect ELISA, immunoblotting and immunoprecipitation. It can also be used in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser308 on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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