EMAIL THIS PAGE TO A FRIEND
D5042 Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

  •  NACRES NA.25

Purchase

Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
Quality Level   100
  200
form   liquid
usage   100 uses (0.1 mg size)
  250 uses (250 μg size)
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

General description

Sigma’s 123 bp DNA Step Ladder contains 35 dsDNA fragments from 123 to 4,182 bp. The fragments are 1 - 34 repeats of a 123 bp region of the rat prolactin gene. Ten ul of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Components

Sigma’s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Other Notes

Contains 35 fragments, 123 - 4182 bp

For optimal resolution, the recommended agarose gel concentration is 1.5%.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Introduction to Markers and Ladders

We offer a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, PAGE, Polymerase chain reaction, Proteomics

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

D3937 DirectLoad 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis
D0428 1 kb DNA Ladder, for DNA electrophoresis
S7025 50 bp DNA Step Ladder, for DNA electrophoresis

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?