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D5652 Sigma

Dulbecco’s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Synonym: DPBS



Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Formulated to contain 9.6 grams of powder per liter of medium.


D-PBS is used to wash cells during preparation and serial transfer.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Price and Availability

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?
Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.
Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?
Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use.  It can be autoclaved at 121°C, 15 psi for 20 minutes.
Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?
Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.
What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?
This product contains 8.0 g/L sodium chloride.  The complete formulation can be found at: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/generalinformation2/dpbs.Par.0001.File.tmp/dpbs.pdf
What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?
The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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