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D5695 Sigma

Anti-DUT (46-60) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Deoxyuridine 5′-triphosphate nucleotidohydrolase, isoform DUT-N mitochondrial precursor, Anti-dUTPase



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to DNA Repair Proteins,
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~18 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DUT(1854)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 46-60 of human DUT

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-DUT antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:500-1:2000. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

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Biochem/physiol Actions

The gene encodes a protein that forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. The dUMP that is formed serves as a precursor for the synthesis of thymine nucleotides needed for DNA replication. It limits intracellular pools of dUTP. It is critical for the fidelity of DNA replication and repair.

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Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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