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  • D6569 - Anti-DBP5 (C-terminal) antibody produced in rabbit

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D6569 Sigma-Aldrich

Anti-DBP5 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DDX19B, Anti-DEAD (Asp-Glu-Ala-As) box polypeptide 19B, Anti-DEAD box RNA helicase, Anti-DEAD box protein 19B, Anti-DEAD5

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for RNA Regulation and Translation, Antibodies to Ribosomal Proteins and Translation Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μL using HEK-293T cell lysate
  indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human DBP5
  western blot: 0.5-1 μg/mL using HEK-293T cell lysate
species reactivity   human, mouse, rat
mol wt   antigen ~53 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9UMR2
Gene Information   human ... DDX19B(11269)
mouse ... Ddx19b(234733)

Description

General description

DBP5 belongs to the family of the DEAD-box helicases that are involved in cellular RNA metabolism from transcription through pre-mRNA splicing, nuclear export, translation initiation to RNA degradation. It localizes within the cytoplasm and at the nuclear rim, where it interacts with components of the nuclear pore complex (NPC). It shuttles between the nucleus and the cytoplasm by using Nup159 as a binding platform.

Application

Anti-DBP5 antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μL using HEK-293T cell lysate, immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cell lysate and immunofluorescence at a working concentration of 2.5-5μg/mL using paraformaldehyde fixed HEK-293T cells over-expressing human DBP5.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

DBP5 is required for mRNA export from the nucleus in an ATP-dependent manner. It is also involved in translation termination, where it recognizes stop-codon by controlling eRF1-eRF3 interaction.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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