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  • D6665 - 2′,7′-Dichlorofluorescein

D6665 Sigma-Aldrich


~90% (TLC), crystalline

  • CAS Number 76-54-0

  • Empirical Formula (Hill Notation) C20H10Cl2O5

  • Molecular Weight 401.20

  •  Beilstein/REAXYS Number 58009

  •  EC Number 200-968-6

  •  MDL number MFCD00005047

  •  PubChem Substance ID 24894041

  •  NACRES NA.47



Related Categories Cell Biology, D, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
assay   ~90% (TLC)
form   crystalline
application(s)   hematology: suitable
  histology: suitable
mp   280 °C (dec.) (lit.)
solubility   ethanol: 25 mg/mL
density   0.79 g/cm3
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI   1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H



2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Biochem/physiol Actions

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What can be used to make a solution of 2´,7´-dichlorofluorescein?
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
In what types of applications can 2´,7´-Dichlorofluorescein be used?
Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.See G. Gbitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as afluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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