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  • D6815 - 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

D6815 Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

for Membrane Applications




3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
• in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
• in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
• in immunoperoxidase staining of lung tissue

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.


Set consists of 4 x 100 ml Solution A and 15 ml Solution B.


Stable for up to 18 mo. when stored at 2-8 °C in the original container.


Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

Kit component only


Product #

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3, 3′-Diaminobenzidine enhanced liquid substrate system, Solution B 15 mL    
3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100 mL    
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
NONH for all modes of transport


Certificate of Analysis


Certificate of Origin

Protocols & Articles


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