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D7006 Sigma-Aldrich

Dihydrofolic acid

≥90%

Synonym: 7,8-Dihydropteroyl-L-glutamic acid, Dihydropteroyl-L-glutamic acid, FAH2

  • CAS Number 4033-27-6

  • Empirical Formula (Hill Notation) C19H21N7O6

  • Molecular Weight 443.41

  •  Beilstein/REAXYS Number 69017

  •  MDL number MFCD00083619

  •  PubChem Substance ID 24894068

  •  NACRES NA.79

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cofactors,
biological source   synthetic (organic)
InChI Key   OZRNSSUDZOLUSN-LBPRGKRZSA-N
assay   ≥90%
form   powder
color   light yellow to dark yellow-orange
storage temp.   −20°C
Gene Information   human ... DHFRP1(573971)
mouse ... Dhfr(13361)

Description

General description

Intermediate in mammalian conversion of dietary folic acid to tetrahydrofolate by dihydrofolate reductase (DHFR). In bacteria, dihydrofolic acid is generated from 7,8-dihydropteroate by dihydrofolate synthetase.

Application

Dihydrofolic acid has been used in diaphorase-coupled assay for dihydrofolate reductase (DHFR) activity. It has also been used as a component in assay buffer to determine dihydrofolate reductase (DHFR) activity.

Biochem/physiol Actions

Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon’ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).

Packaging

Sealed ampule.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. D7006.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Folic Acid Metabolism: A Role in Cancer's Cause and Cure

The mammalian folic acid cycle is a highly complex but crucial process for the transfer of one-carbon units to amino acids, nucleotides, and other biomolecules. Folic acid cannot be synthesized but i...
Biofiles Vol. 5, No. 6
Keywords: Antibiotics, Antitumor agents, Apoptosis, Building blocks, Cancer, Cellular processes, Eliminations, Gastrointestinal, Metabolic Pathways, Metabolism, Metabolites, Methylations, Phosphorylations, Purine synthesis, Reductions, transformation

Peer-Reviewed Papers
15

References

Related Products

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CS0340 Dihydrofolate Reductase Assay Kit,  kit sufficient for 50-100 tests
06693 Timestrip Plus -20 °C

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