• USA Home
  • D7052 - Dipeptidyl Peptidase IV from porcine kidney

D7052 Sigma

Dipeptidyl Peptidase IV from porcine kidney

lyophilized powder, ≥10 units/mg protein (Bradford)

Synonym: Dipeptidyl aminopeptidase IV



Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Dipeptidyl peptidase IV,
form   lyophilized powder
packaging   vial of ≥0.75 unit
solubility   0.1 M Tris-HCl, pH 8.0: soluble 1 vial/mL, clear, colorless
storage temp.   −20°C
Gene Information   pig ... DPPIV(397492)


Biochem/physiol Actions

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

The pH optimum for the protease is 7.8-8.0. Dipeptidyl peptidase does not require activators. It is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, and diethyl p-nitrophenyl phosphate.


DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline.

Unit Definition

One unit will produce 1.0 μmole of p-nitroaniline from Gly-L-Pro p-nitroanilide per min in 100 mM Tris-HCl at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts


Dipeptidyl peptidase IV from porcine kidney has been used in a study to investigate engraftment of donor cells following hepatocyte transplantation. Dipeptidyl peptidase IV from porcine kidney has also been used in a study to investigate the metabolism of glucagon-like peptide-2.

The enzyme from sigma has been used in the determination of DPP-IV inhibitory activity of peptides in porcine skin gelatin hydrolysates.

Price and Availability

Expore Enzymes, Proteins & Cofactors

Celebrate Pi Day!
Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products


Product #


Add to Cart

K4264 KR-62436 hydrate, ≥95%, powder
D0879 Diisopropylfluorophosphate
P7626 Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
I9759 Ile-Pro-Ile, ≥97% (HPLC)


Product #


Add to Cart

G2901 Gly-Pro p-nitroanilide p-toluenesulfonate salt, ~99% (TLC)
G2761 Gly-Pro-7-amido-4-methylcoumarin hydrobromide, dipeptidylpeptidase IV substrate
G9262 Gly-Pro 4-methoxy-β-naphthylamide hydrochloride, powder
G9137 Gly-Pro 4-methoxy-β-naphthylamide, dipeptidylpeptidase IV substrate
G0513 Gly-Pro p-nitroanilide hydrochloride, ≥99% (HPLC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?