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D7295 Sigma

Deoxynucleotide Mix, 10 mM

Molecular Biology Reagent

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Properties

Related Categories 10 mM Solutions, Biochemicals and Reagents, Core Bioreagents, Deoxynucleotides, General Reagents,
form   liquid
concentration   10 mM
color   colorless
Featured Industry   Agriculture
foreign activity   DNase, RNase, none detected
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP and TTP sodium salts in high quality molecular biology grade water. One μL is sufficient for a standard 50 μL PCR reaction.

Suitable for routine and long PCR, manual and automated DNA sequencing, cDNA synthesis and labeling reactions.

Deoxynucleotide Mix, 10mM has been used during DNA amplification by PCR.

Features and Benefits

• Purity of each dNTP: Minimum 99%
• Conveniently formulated; 1 μL is used per 50 μL PCR
• Equimolar amounts of each dNTP means less pipetting
• Minimize risk of contamination in PCR
• UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

Packaging

0.2, 20×0.2, 0.5 mL in poly bottle

General description

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide.

Price and Availability


KiCqStart Probe Assays

PCR Selection Tool

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What are the components to Product D7295, Deoxynucleotide Mix?
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water.  This is for a total concentration of 40 mM.
Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
The product contains Thymidine 5′-triphosphate sodium salt.  Thymidine has a deoxy sugar, so it is a deoxythymidine.  
How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
One μl is sufficient for a standard 50 μl PCR reaction.
Show more questions
Protocols & Articles

Articles

Transplex Whole Transcriptome RNA Amplifications

The efficacy of amplification of small quantities of total RNA with the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasin...
Ken Heuermann, Brian Ward
Biowire Volume 10 Article 1
Keywords: AGE, Amplification, Applications, Cancer, Clinical, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genomics, Instructions, Marketing, Methods, Microarray Analysis, Microbiology, Nucleic acid annealing, Nucleic acid hybridization, Oligonucleotide microarrays, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Reductions, Transcription

Protocols

Amplification of DNA Using Jumpstart Taq DNA Polymerase D9307 Protocol

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase

Note: The use of DMSO or formamide with JumpStart REDTaq DNA Polymerase is not recommended due to interference with the enzyme-antibody complex. Other co-solvents, solutes (e.g., salts) and extremes ...
Keywords: Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase

Note: JumpStart Taq DNA polymerase has been shown to work effectively with up to 5% v/v DMSO. Other co-solvents, solutes (e.g., salts) and extremes in pH or other reaction conditions may reduce the a...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Solvents, Titrations

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

Restorase® DNA Polymerase with 10x Reaction Buffer combines Sigma's Long and Accurate enzyme technology with a DNA repair enzyme resulting in a blend that facilitates repair and amplification of dama...
Keywords: AGE, Alkylations, Amplification, Buffers, Catalysis, Degradations, Diagnostic, Electrophoresis, Gas chromatography, Gel electrophoresis, Melting, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Precipitation, Size-exclusion chromatography

Amplification of Genomic DNA using REDTaq DNA Polymerase

REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplificatio...
Keywords: AGE, Amplification, Applications, Buffers, Digestions, Electrophoresis, Evaporation, Formulations, Gel electrophoresis, Polymerase chain reaction, Purification, Sequencing, transformation

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References
Keywords: Amplification, Buffers, Cloning, Digestions, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Reductions, Sequencing, Size-exclusion chromatography, transformation

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Long and Accurate PCR Amplification of DNA D8045 Protocol

Effective denaturation is accomplished by the use of higher temperatures for shorter periods of time or by the use of co-solvents, such as dimethyl sulfoxide. Addition of DMSO in the reaction at a fi...
Keywords: AGE, Amplification, Buffers, Diagnostic, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Melting, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Size-exclusion chromatography, Solvents

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...
Keywords: AGE, Amplification, Buffers, Diagnostic, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Melting, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Size-exclusion chromatography, Solvents

Low Contaminant Amplification of DNA Using MTP Taq DNA Polymerase D7442 Protocol

Every precaution should be taken to avoid contamination of reagents with unknown/unwanted DNA. This includes the following:
Keywords: AGE, Amplification, Buffers, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme h...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, Gene expression, PAGE, Polymerase chain reaction

Standard PCR protocol

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It is able to withstand repeated heat...
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

Related Content

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Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Protein Tyrosine Kinase and Phosphatase Expression Profiling - Cancer Research

By focusing on analysis techniques and protocols specific for Cancer Research, Sigma-Aldrich® is putting your research needs first. We will be featuring different cancer research tools throughout the...
Keywords: Amplification, Antibiotics, Cancer, Catalysis, Cell signaling, Cloning, Digestions, Electrophoresis, Enzyme activity, Filtration, Gel electrophoresis, Gene expression, Molecular biology, Nucleic acid denaturation, Nucleic acid hybridization, Peptide synthesis, Polymerase chain reaction, Polymorphisms, Purification, Separation, Sequencing, Transcription, transformation

Peer-Reviewed Papers
15

References

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