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D7304 Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride

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Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Caution

Stable at least one year at 2-8 °C.

Quantity

Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.

Application

DAB Liquid Substrate System is a precipitating substrate system for peroxidase. It produces a brown precipitate for use in immunohistological and immunoblotting staining procedures. Not recommended for ELISA (microwell) procedures. DAB has been used as a substrate in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver. It has been used as a substrate for visualization of sections after immunostaining.

Standard component

Description

Product #

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10X DAB Liquid Chromogen 25 mL    
ready-to-use buffer/peroxide solution 250 mL    
Safety & Documentation

Safety Information

Symbol 
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Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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