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D7810 Sigma

Anti-Daxx antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Adaptors,
species reactivity   monkey, mouse, human
application(s)   microarray: suitable
  western blot: 1:4,000 using human acute lymphoma Jurkat whole cell extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 120 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DAXX(1616)
mouse ... Daxx(13163)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to the C-terminus of human Daxx, (amino acids 721-740). This sequence is identical in mouse and monkey.

General description

The death domain-associated protein (Daxx) is a protein that interacts with FAS and subsequently regulates cellular apoptosis . Daxx also interacts with H3.3 histone and ATRX to regulate chromatin deposition at telomeres . Anti-Daxx is specific for human Daxx (120 kDa). In immunoblotting, staining of Daxx is specifically inhibited with the Daxx immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-Daxx antibody is suitable for use in western blot (1:4,000 using human acute lymphoma Jurkat whole cell extract), immunoblot and microarray.

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Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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