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D8130 Sigma-Aldrich

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Synonym: 3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

  • CAS Number 69-78-3

  • Linear Formula [-SC6H3(NO2)CO2H]2

  • Molecular Weight 396.35

  •  Beilstein/REAXYS Number 1896821

  •  EC Number 200-714-4

  •  MDL number MFCD00007140

  •  PubChem Substance ID 24894189

  •  NACRES NA.26

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Properties

Related Categories Amino Acid Analysis, Amino Acid Analysis Reagents, Analytical and Industrial Enzymes, Biochemicals and Reagents, Chemical Synthesis,
Quality Level   200
product line   BioReagent
assay   ≥98%
mp   240-245 °C (dec.) (lit.)
suitability   suitable for determination of sulfhydryl groups
greener alternative category   Re-engineered
SMILES string   OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O
InChI   1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChI key   KIUMMUBSPKGMOY-UHFFFAOYSA-N

Description

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

Packaging

1, 5, 10, 25, 100 g in poly bottle

500 mg in glass insert

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
DG9650000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To standardize a procedure for the determination of the enzymatic assay of Chloramphenicol Acetyltransferase.
Keywords: Enzyme activity, Enzymology, Extinction coefficient

Peer-Reviewed Papers
15

References

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