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  • D8168 - Monoclonal Anti-Dystrophin antibody produced in mouse

D8168 Sigma-Aldrich

Monoclonal Anti-Dystrophin antibody produced in mouse

clone MANDYS8, ascites fluid

Synonym: Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse, Dystrophin Antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   MANDYS8, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
  microarray: suitable
  western blot: suitable
species reactivity   rat, chicken, mouse, pig, human, rabbit
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2b
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P11532
Gene Information   human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)


General description

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.


recombinant human dystrophin fragment.


Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
• immunohistochemistry
• immunofluorescence
• double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
• immunoblotting

Target description

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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