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  • D8168 - Monoclonal Anti-Dystrophin antibody produced in mouse

D8168 Sigma

Monoclonal Anti-Dystrophin antibody produced in mouse

clone MANDYS8, ascites fluid

Synonym: Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse - Tested Applications: immunoblotting,indirect ELISA,array,indirect immunofluorescence frozen human or animal muscle tissue sections. 1:400 - Sigma Aldrich D8168, Dystrophin Antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   rat, chicken, mouse, pig, rabbit, human
application(s)   indirect ELISA: suitable
  indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
  microarray: suitable
  western blot: suitable
clone   MANDYS8, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)
biological source   mouse
conjugate   unconjugated



recombinant human dystrophin fragment.

General description

Dystrophin is a 427kD cytoskeletal protein found in muscle sarcolemma as a membrane-spanning protein complex which connects the cytoskeleton to the basal lamina. Defects in the gene coding for dystrophin leads to X-linked Duchenne muscular dystrophy. Monoclonal anti-dystrophin antibody can be used to study the structure and function of dystrophin and its interactions with other proteins. The antibody can also be used in immunohistochemical analysis for prenatal or post-abortion diagnosis of muscle dystrophies carriers. Mouse monoclonal anti-dystrophin antibody reacts specifically with an epitope present on the rod domain of the human dystrophin molecule.

Antigen Background

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry-paraffin (1 paper)

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

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RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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