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  • D9281 - Lambda DNA EcoR I Hind III Digest

D9281 Sigma-Aldrich

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

  •  NACRES NA.25



Related Categories DNA Digest Markers, Markers and Ladders, Molecular Biology, Nucleic Acid Electrophoresis
Quality Level   100
form   liquid
usage   100 uses (0.1 mg size)
  500 uses (0.5 mg size)
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C


General description

Sigma’s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.


Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.


Sigma’s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Introduction to Markers and Ladders

We offer a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, PAGE, Polymerase chain reaction, Proteomics

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction


Experienced User Protocol | GenElut Mammalian Genomic DNA Miniprep Kit

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: PAGE

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute™ Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whole b...
Keywords: AGE, Amplification, Anticoagulants, Cell disruption, Centrifugation, DNA purification, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sample preparations, Sequencing

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