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  • D9442 - Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit

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D9442 Sigma-Aldrich

Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-DAT

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Neurotransmission, Antibodies to Drug Transport Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: 1:1,000 using formaldehyde-fixed human and monkey brain sections
  western blot: 1:1,000 using SDS-solubilized human striatal samples
species reactivity   human, monkey
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q01959
Gene Information   human ... SLC6A3(6531)

Description

Immunogen

synthetic peptide from the extracellular loop 2 region of human dopamine transporter.

Application

Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit is suitable at a working dilution of 1:1000 for western blotting using SDS-solubilized human striatal samples and for immunohistochemistry using formaldehyde-fixed human and monkey brain sections.

Target description

The dopamine transporter (DAT1) acts to take released dopamine back up into presynaptic terminals and has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse.

Physical form

Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The dopamine transporter (DAT1) acts to take released dopamine back up into presynaptic terminals and has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse. DAT1 gene is a primary target gene for ADHD (Attention deficit hyperactivity disorder). DAT polymorphism along with maternal prenatal smoking is associated with childhood hyperactivity-impulsivity and inattentiveness.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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HPA013602 Anti-DAT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
D6944 Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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