EMAIL THIS PAGE TO A FRIEND
D9542 Sigma-Aldrich

DAPI

for nucleic acid staining

Synonym: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI dihydrochloride

  • CAS Number 28718-90-3

  • Empirical Formula (Hill Notation) C16H15N5 · 2HCl

  • Molecular Weight 350.25

  •  Beilstein/REAXYS Number 4894417

  •  EC Number 249-186-7

  •  MDL number MFCD00012681

  •  PubChem Substance ID 24894305

  •  NACRES NA.52

Purchase

Properties

Related Categories Apoptosis Detection, Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Fluorescent,
grade   for molecular biology
InChI Key   FPNZBYLXNYPRLR-UHFFFAOYSA-N
assay   ≥98% (HPLC and TLC)
form   powder
solubility   H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
  PBS: insoluble
ε (extinction coefficient)   30 at 263 nm in H2O at 1 mM
fluorescence   λex 340 nm; λem 488 nm (nur DAPI)
  λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability   suitable for fluorescence
storage temp.   2-8°C

Description

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for
• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells

DAPI has been used:-
• in rapid monitoring of microbial contamination
• in chromosomal banding technique
• in detection of apoptotic cells
• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
• to stain mature pollen grains(0.5 mg/ml)

Packaging

1, 5, 10, 50 mg in glass bottle

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
This product is soluble in water at 20 mg/mL, but may require heat.DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation.At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks.For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
Aside from the applications described on the product page, how isD9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high.It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the stability of D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride?
Product D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride has a stability of 3 years as supplied. The product in solution is also stable in the dark at 4°C and showed no signs of degradation after three weeks at room temperature in the dark. Material exposed to light showed significant degradation. We have developed an HPLC method for our DAPI products that shows reassay results from July 2012 for lots that were originally released for sale in December 2008, for both D9542 (DAPI dihydrochloride) and D9564 (DAPI dilactate).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

LentiBrite™ for fluorescent cell imaging for Cytoskeleton Structure

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

LentiBrite™ for fluorescent cell imaging

Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infecti...
Keywords: Autophagy, Cancer, Diseases, Flow cytometry, Fluorescent microscopy, Gene expression, Immunofluorescence, Microscopy, Tagged proteins, Transduction, Transfection

Live Cell Cycle Assay

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associa...
Kevin Su1, Nick Asbrock1, Vi Chu1, Tomas Kraus2
1MilliporeSigma, Temecula, CA; 2Institute of Organic Chemistry and Biochemistry AS CR, Czech Republic
Keywords: Building blocks, Cancer, Cell culture, Cell division, Cell proliferation, Centrifugation, Confocal microscopy, Culture media, Enzyme-linked immunosorbent assay, Flow cytometry, Genetic, Microscopy, Peptide synthesis

Protocols

Fluorescent in situ Hybridization (FISH)

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Nucleic acid hybridization, Polymerase chain reaction

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?