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  • D9693 - Anti-Doublecortin antibody produced in rabbit

D9693 Sigma

Anti-Doublecortin antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DCX, Anti-Doublecortex, Anti-LISX, Anti-X-linked lissencephaly, Anti-XLIS



Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, DM-DP,
species reactivity   mouse, human, rat
application(s)   western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction).
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~40 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... DCX(1641)
mouse ... Dcx(13193)
rat ... Dcx(84394)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 151-170 of human doublecortin, conjugated to KLH. The corresponding sequence is identical in rat and mouse doublecortin.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cells expressing human doublecortin, and mouse brain and rat brain extract (S1 fraction).

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Biochem/physiol Actions

Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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D9943 Anti-Doublecortin (N-terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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