D9891 Sigma

Doxycycline hyclate

≥98% (HPLC)

Synonym: Doxycycline hydrochloride hemiethanolate hemihydrate




Do you have application information on this product that you would like to share?

Doxycycline hyclate is a synthetic oxytetracycline derivative. It has been used to eliminate Borrelia burgdorferi and Anaplasma phagocytophilum in rodent reservoirs and to eliminate Ixodes scapularis ticks. It is a broad spectrum inhibitor used to inhibit matrix metalloproteinases (MMP), such as type 1 collagenase in studies on wound healing and tissue remodeling.


1, 5, 10, 25, 100 g in glass bottle

Biochem/physiol Actions

Doxycycline hyclate inhibits the inflammatory response to the Lyme Disease Spirochete Borrelia burgdorferi. It is a broad spectrum inhibitor of matrix metalloproteinases in vivo.

General description

Chemical structure: tetracycline

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Bulk Chemicals

LC-MS Grade Solvents and Reagents

Celebrate Pi Day!
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

The name of Product D9891 is Doxycycline hyclate. What does hyclate mean?
Hyclate is the United States Adopted Names (USAN) approved contraction for monohydrochloride hemiethanolate hemihydrate.
What is the solubility of Doxycycline hyclate, Product D9891?
Doxycycline hyclate is soluble in water at up to 50 mg/ml. To view this information in D9891 Product Information sheet, click on the following link:
How stable is Doxycycline hyclate, Product D9891, in water?
Solutions of doxycycline hyclate are best prepared fresh, but they can be stored at 2-8°C for several days. Solutions also need to be protected from light. This information is also available from the product information sheet, which is available via the following link:
What type of activity does Product D9891, Doxycycline hyclate, exhibit?
Doxycycline is a broad spectrum antibiotic and bacteriostatic agent against gram positive/negative bacteria and it has antiprotozoal activity. To view this information in D9891 Product Information sheet, click on the following link:
At what concentration should I use D9891, Doxyclycline hyclate, for cell culture?
Doxycycline is often used in culture at a concentration of 0.1-12 µg/ml. To view this information in D9891 Product Information sheet, click on the following link:
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Is doxycycline hyclate soluble in DMSO?
According to literature sources, doxycycline hyclate is soluble in DMSO at a concentration of 100 mM.
Show more questions
Protocols & Articles

Related Content

Bio Brief: Doxycycline Hyclate [VIDEO]

Doxycycline hyclate is a semisynthetic tetracycline functioning as a broad spectrum antibiotic and bacteriostatic agent effective against Gram-positive and Gram-negative bacteria. The recommended usa...
Keywords: Antibiotics, Apoptosis, Cancer, Cell culture, Gene expression, Gene knockdown

Peer-Reviewed Papers


Related Products

also commonly purchased with this product

Product #


Add to Cart

Z672548 PAP pen for immunostaining, 2 mm tip width
O5875 Oxytetracycline hydrochloride, ≥95% (HPLC), crystalline
D6140 Demeclocycline hydrochloride, ≥90% (HPLC), powder
M9511 Minocycline hydrochloride, crystalline
P8833 Puromycin dihydrochloride from Streptomyces alboniger, powder, BioReagent, suitable for cell culture

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?