D9891 Sigma-Aldrich

Doxycycline hyclate

Synonym: Doxycycline hydrochloride hemiethanolate hemihydrate

  • CAS Number 24390-14-5

  • Empirical Formula (Hill Notation) C22H24N2O8 · HCl · 0.5H2O · 0.5C2H6O

  • Molecular Weight 512.94

  •  Beilstein Registry Number 5702728

  •  MDL number MFCD07357237

  •  PubChem Substance ID 57654124




1, 5, 10, 25, 100 g in glass bottle


Doxycycline hyclate has been used for the induction of glioma cells and human embryonic kidney 293T cells (HEK293T).

Doxycycline hyclate is a synthetic oxytetracycline derivative. It has been used to eliminate Borrelia burgdorferi and Anaplasma phagocytophilum in rodent reservoirs and to eliminate Ixodes scapularis ticks. It is a broad spectrum inhibitor used to inhibit matrix metalloproteinases (MMP), such as type 1 collagenase in studies on wound healing and tissue remodeling.

Biochem/physiol Actions

Doxycycline hyclate inhibits the inflammatory response to the Lyme Disease Spirochete Borrelia burgdorferi. It is a broad spectrum inhibitor of matrix metalloproteinases in vivo.

General description

Chemical structure: tetracycline

Doxycycline hyclate belongs to the second generation of tetracycline antibiotics family. Doxycycline hyclate shows minimal side effects upon usage. It is effective for the treatment of recurrent aphthous stomatitis (RAS) and promotes speedy recovery.

Other Notes

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Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

The name of Product D9891 is Doxycycline hyclate. What does hyclate mean?
Hyclate is the United States Adopted Names (USAN) approved contraction for monohydrochloride hemiethanolate hemihydrate.
What is the solubility of Doxycycline hyclate, Product D9891?
Doxycycline hyclate is soluble in water at up to 50 mg/ml.
How stable is Doxycycline hyclate, Product D9891,in water?
Solutions of doxycycline hyclate are best prepared fresh, but they can be stored at 2-8°C for several days. Solutions also need to be protected from light.
What type of activity does Product D9891, Doxycycline hyclate, exhibit?
Doxycycline is a broad spectrum antibiotic and bacteriostatic agent against gram positive/negative bacteria and it has antiprotozoal activity.
At what concentration should I useD9891, Doxyclycline hyclate, for cell culture?
Doxycycline is often used in culture at a concentration of 0.1-12 μg/ml.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Is doxycycline hyclate soluble in DMSO?
According to literature sources, doxycycline hyclate is soluble in DMSO at a concentration of 100 mM.
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