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D9905 Sigma-Aldrich

Denhardt’s Solution

for Northern and Southern blotting, lyophilized powder



Related Categories Biochemicals, Blocking Reagents for Hybridization, Core Bioreagents, General Reagents, Life Science Reagents for Northern and Southern Blotting,
grade   for molecular biology
form   lyophilized powder
foreign activity   DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.   2-8°C


General description

Denhardt’s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.


Denhardt′s Solution has been used:
• as a component in hybridization buffer for the hybridization of brain sections
• in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
• in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.


5 mL in glass bottle

Biochem/physiol Actions

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.


Each vial of Denhardt’s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


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