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  • D9943 - Anti-Doublecortin (N-terminal) antibody produced in rabbit

D9943 Sigma-Aldrich

Anti-Doublecortin (N-terminal) antibody produced in rabbit

Antibody Enhanced Validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-DCX, Anti-Doublecortex, Anti-LISX, Anti-X-linked lissencephaly, Anti-XLIS

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, DM-DP,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction)
  western blot: 1-2 μg/mL using mouse brain extract (S1 fraction)
species reactivity   mouse, human, rat
mol wt   antigen ~40 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   O43602
Gene Information   human ... DCX(1641)
mouse ... Dcx(13193)
rat ... Dcx(84394)



Anti-Doublecortin(N-terminal) specifically recognizes human, rat, and mouse Doublecortin.


synthetic peptide corresponding to amino acids 1-17 located at the N-terminus of rat doublecortin, conjugated to KLH. This sequence is identical in mouse, and highly conserved in human doublecortin.


Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), and 1-2μg/mL using mouse brain extract (S1 fraction).

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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D9693 Anti-Doublecortin antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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