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DNTPCA1 Sigma-Aldrich

CleanAmp dNTP

Hot-start dNTP mix for improved PCR

Synonym: Deoxynucleotides, dNTP Mix, dNTP

Purchase

Properties

Related Categories Molecular Biology, PCR/Amplification, dNTP Mix, dNTPs (Deoxynucleotides)
form   liquid
feature   hotstart
  hotstart
concentration   10 mM (each dNTP)
color   colorless
shipped in   dry ice
storage temp.   −20°C

Description

Application

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers
• Substitute for dNTPs in any PCR reaction

General description

CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Sigma Life Science is proud to offer new, improved, CleanAmp dNTPs. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Packaging

2 μmoles: 1 vial, 200 μL, each dNTP at 10 mM in 50 mM Glycine
10 μmoles: 1 vial, 1000 μL, each dNTP at 10 mM in 50 mM Glycine

Legal Information

CleanAmp dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer’s right to have and use the CleanAmp dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.

CleanAmp is a trademark of TriLink BioTechnologies, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Polymerase Chain Reaction - PCR Technologies Guide

The polymerase chain reaction (PCR)1,2,3 has become one of the most widely used techniques in molecular biology. It is used in applications from basic research to high-throughput screening. While it ...
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Quantitative PCR - PCR Technologies Guide

As described in Introduction and Historical Timelines and Polymerase Chain Reaction, PCR is currently considered to be the most useful technique that is applied to investigations using molecular biol...
Keywords: Amplification, Buffers, Clinical, Clinical Chemistry, Detection methods, Electrophoresis, Enzyme activity, Gel electrophoresis, Gene expression, Melting, Methylations, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, PAGE, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Size-exclusion chromatography, Transcription

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References
Keywords: Amplification, Buffers, Cloning, Digestions, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Reductions, Sequencing, Size-exclusion chromatography, transformation

Hot Start dNTP protocol to reduce non-specific amplification

· How do Hot-Start dNTPs work? · Handling · Protocols for Taq DNA Polymerase—Standard PCR, Fast PCR, Multiplexed PCR and Real-Time PCR · Troubleshooting · Standard Thermal Cycling Conditions for Othe...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography

Related Content

PCR Selection Guide

We offer a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from routine to enh...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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Add to Cart

DNTPCA2 CleanAmp dNTP, Modified dNTP set for hot-start PCR, 2 μmol of each dNTP
DNTPCA10 CleanAmp dNTP, 10 μmol of each dNTP, Modified dNTP set for hot-start PCR
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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