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DNTPCA2 Sigma-Aldrich

CleanAmp dNTP

Modified dNTP set for hot-start PCR, 2 μmol of each dNTP

Synonym: Deoxynucleotides, dNTP Mix, dNTP

Purchase

Properties

Related Categories Deoxynucleotide Sets, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
form   liquid
quality   2 μmol of each dNTP
feature   hotstart
  hotstart
concentration   50 mM (each dNTP)
color   colorless
shipped in   dry ice
storage temp.   −20°C

Description

Application

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers
• Substitute for dNTPs in any PCR reaction

General description

CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Packaging

2 μmoles: 4 vials:
40 μL dATP at 50 mM in buffered glycine solution
40 μL dCTP at 50 mM in buffered glycine solution
40 μL dTTP at 50 mM in buffered glycine solution
40 μL dGTP at 50 mM in buffered glycine solution

Legal Information

CleanAmp dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.

CleanAmp is a trademark of TriLink BioTechnologies, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Hot Start dNTP protocol to reduce non-specific amplification

· How do Hot-Start dNTPs work? · Handling · Protocols for Taq DNA Polymerase—Standard PCR, Fast PCR, Multiplexed PCR and Real-Time PCR · Troubleshooting · Standard Thermal Cycling Conditions for Othe...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecti...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography, Transcription

Related Content

PCR Selection Guide

We offer a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from routine to enh...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

DNTPCA1 CleanAmp dNTP, Hot-start dNTP mix for improved PCR
DNTPCA10 CleanAmp dNTP, 10 μmol of each dNTP, Modified dNTP set for hot-start PCR

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