E0282 Sigma-Aldrich

MaxGel ECM

mixture, liquid

  •  eCl@ss 32190102




MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.


This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.

MaxGel ECM is used for the following applications:
• Extracellular matrix (ECM) protein binding assays
• Cell invasion assay
• Cell culture


MaxGel will remain stable for at least 1 year when stored at -70°C.

Legal Information

MaxGel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
WillProduct E0282, MaxGel ECM, work for adherent cells and suspension cells?
Yes, this product works for both cell types.
How isProduct E0282, MaxGel ECM, supplied?
E0282 is supplied at a concentration of 1 mg/ml in DMEM.
What is an appropriate working concentration for Product E0282, MaxGel ECM?
MaxGel works best undiluted or even diluted 1:21:4.For a thin coat plating method, this material can be diluted as high as 1:100.
How do I handle Product E0282, MaxGel ECM?
Remove the product from the freezer and thaw on ice just prior to use. It quickly forms a gel when allowed to warm to room temperature, so it is necessary to keep the product on ice until coating.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Cell Culture Guide

Same Products - New Packaging
Protocols & Articles


3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

MaxGel™ ECM is an in vitro derived human basement membrane extracts (BME) that provides a rich three-dimensional environment to promote cellular proliferation for multiple cell types. MaxGel™ ECM con...
Keywords: Biomaterials, Cancer, Cell culture, Cell proliferation, Culture media, Diabetes, Diseases, Growth factors, Purification, Reductions

Neural Stem Cell Culture

Neural Stem Cell Lines Neural Stem Cell Media and Supplements Neural Stem Cell Markers Stem Cell Culture Protocols
Keywords: Cell biology, Cell culture, Central Nervous System, Cryopreservation, Diseases, Dopamine agents, Environmental, Gene expression, Growth factors, Neuroscience, Phase transitions, Stem cell biology, Transcription

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?