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E0282 Sigma-Aldrich

MaxGel ECM

mixture, liquid

  •  eCl@ss 32190102

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Description

Components

MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.

Application

This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.

MaxGel ECM is used for the following applications:
• Extracellular matrix (ECM) protein binding assays
• Cell invasion assay
• Cell culture

Caution

MaxGel will remain stable for at least 1 year when stored at -70°C.

Legal Information

MaxGel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
WillProduct E0282, MaxGel ECM, work for adherent cells and suspension cells?
Yes, this product works for both cell types.
How isProduct E0282, MaxGel ECM, supplied?
E0282 is supplied at a concentration of 1 mg/ml in DMEM.
What is an appropriate working concentration for Product E0282, MaxGel ECM?
MaxGel works best undiluted or even diluted 1:21:4.For a thin coat plating method, this material can be diluted as high as 1:100.
How do I handle Product E0282, MaxGel ECM?
Remove the product from the freezer and thaw on ice just prior to use. It quickly forms a gel when allowed to warm to room temperature, so it is necessary to keep the product on ice until coating.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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References

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