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E0412 Sigma-Aldrich

Carboxylesterase 2 human

recombinant, expressed in baculovirus infected BTI insect cells

Synonym: CES2, CES2A1, Carboxylesterase 2 human



Quality Level   200
recombinant   expressed in baculovirus infected BTI insect cells
form   liquid
specific activity   ≥500 units/mg protein

mol wt   60 kDa
weight   (0.5 ml)
shipped in   dry ice
storage temp.   −70°C


General description

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.

Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human. It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.


Carboxylesterase 2 human has been used:
•  in the in vitro enzyme-based inhibitor screening assay
• in substrate selectivity assay with emission ratiometric two-photon probe (SE1)
• as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.

Biochem/physiol Actions

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group. It shows less transesterification activity due to presence of conformational interference in active site.

Member of a serine esterase family that hydrolyze ester and amide bonds. Carboxylesterase 2 is an endoplasmic reticulum-bound hydrolase that plays a critical role in xenobiotic detoxification and activation for ester-containing therapeutics. Carboxylesterase 2 is also involved in the detoxification of drugs such as heroin and cocaine. This enzyme is thought to play a role in lipid metabolism.

Unit Definition

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers


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