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  • E0521 - Anti-Estrogen Receptor (18-32) antibody produced in rabbit

E0521 Sigma

Anti-Estrogen Receptor (18-32) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Hormones, Antibodies to Steroid Hormones and Related Products,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:3000 using MCF7 cultured cell line extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 67 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ESR1(2099)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 18-32 of the human estrogen receptor.

General description

Estrogen is a sex hormone that is a key regulator of growth, differentiation and function of reproductive tissues, skeletal and cardiovascular systems. The effect of estrogen is mediated by two intracellular receptors, ERα and ERβ. Although the two receptors interact with similar ligands, have identical DNA-binding domains and have redundant roles in estrogen signaling, they show distinct expression pattern. On binding to the receptor, the estrogen-ER complex enters nucleus and regulates the transcription of specific genes. The biological effects of estrogen and ER is by upstream activation of the pathway by growth factors. Some pathways that activate the estrogen-ER pathway are those mediated by AP-1, IGF-1, EGF, MAPK and PI3K/Akt . Anti-Estrogen Receptor (18-32) antibody is specific for 18-32 amino acids of human estrogen receptor (67 kDa). The product is an IgG fraction prepared from pooled antisera.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.


Anti-Estrogen Receptor (18-32) antibody may be used for immunoblotting using extracts of MCF7 cells at a working dilution of 1:3000. It may also be used for dot blot.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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