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E0532 Sigma-Aldrich

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 102618, purified immunoglobulin, lyophilized powder

Synonym: Anti-EGFR

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Epidermal Growth Factors (EGF) and NRG Family,
conjugate   unconjugated
clone   102618, monoclonal
biological source   mouse
application(s)   western blot: 1-2 μg/mL
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
UniProt accession no.   P00533
Gene Information   human ... EGFR(1956)

Description

General description

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis
Monoclonal anti-EGF Receptor detects human EGF receptor. This antibody shows no cross-reactivity with recombinant mouse EGF R, recombinant human ErbB2, recombinant mouse ErbB2, recombinant human ErbB3, or recombinant human ErbB4.

Immunogen

purified, NSO cell-derived recombinant human epidermal growth factor receptor extracellular domain.

Application

Anti-EGF R antibody may be used at a working concentration of 1-2 μg/ml for detection of human EGF R by immunoblotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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E1282 Anti-Epidermal Growth Factor Receptor antibody produced in goat, affinity isolated antibody, lyophilized powder
E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 29.1, ascites fluid
E3641 Epidermal Growth Factor Receptor human, buffered aqueous glycerol solution, 5,000-30,000 units/mg protein (Lowry)
E3138 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone F4, ascites fluid
E2645 Epidermal Growth Factor Receptor human, lyophilized powder, ≥15,000 units/mg protein (Bradford)

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