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  • E1530 - Anti-phospho-Epidermal Growth Factor Receptor (pTyr1086) antibody produced in rabbit

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E1530 Sigma-Aldrich

Anti-phospho-Epidermal Growth Factor Receptor (pTyr1086) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-EGFR (pTyr1086)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000
species reactivity   human, mouse, rat
mol wt   antigen ~185 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P00533
Gene Information   human ... EGFR(1956)
mouse ... Egfr(13649)

Description

Immunogen

synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 1086.

Physical form

Solution in Dulbecco′s phosphate buffered saline without Mg2+ and Ca2+, pH 7.3 with 50% glycerol, 1 mg/mL BSA (protease and IgG free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer. Tyr1086 is a minor autophosphorylation site and enables the internalization of the receptor.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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