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  • E1783 - Monoclonal Anti-eIF5A antibody produced in mouse

E1783 Sigma

Monoclonal Anti-eIF5A antibody produced in mouse

~1.0 mg/mL, clone IF5-88, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Eukaryotic translation initiation factor 5A, Anti-eIF-4D, Anti-eIF-5A-1, Anti-eIF-5A1



Related Categories Alphabetical Index, Antibodies, E-EI, Primary Antibodies
species reactivity   rat, mouse, canine, human, monkey
application(s)   immunocytochemistry: suitable
  western blot: 1-2 μg/mL using 3T3 total cell extract
clone   IF5-88, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~17 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EIF5A(1984)
mouse ... Eif5a(276770)
rat ... Eif5a(287444)
biological source   mouse
conjugate   unconjugated



recombinant protein corresponding to amino acids 2-70 of human eIF5A.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Monoclonal Anti-eIF5A antibody produced in mouse is suitable for immunocytochemistry and western blot analysis at a working concentration of 1-2μg/mL using 3T3 total cell extract.

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Biochem/physiol Actions

EIF5A (Eukaryotic translation initiation factor 5A-1) gene encodes a mRNA-binding protein involved in translation elongation. It also has an important role in mRNA turnover, mRNA decay, actin dynamics and cell cycle progression. It is also implicated in cell proliferation, synthesis of proteins involved in cell cycle control, and transport of viral mRNAs from nucleus to cytoplasm in association with Rev HIV-I protein. Higher eIF5A protein expression levels are present in lung adenocarcinomas tumors, suggesting eIF5A as a prognostic marker in lung carcinomas. It is a highly conserved protein that undergoes a unique and essential post-translational modification dependent on the polyamine spermidine, called hypusination. Reduction of eIF5A content is implicated in brain aging.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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