E2129 Sigma-Aldrich

Evans Blue

Dye content ≥75 %

Synonym: Direct Blue 53

  • CAS Number 314-13-6

  • Empirical Formula (Hill Notation) C34H24N6Na4O14S4

  • Molecular Weight 960.81

  •  Colour Index Number 23860

  •  EC Number 206-242-5

  •  MDL number MFCD00004021

  •  PubChem Substance ID 24894456

  •  NACRES NA.47



Related Categories Cell Biology, Core Bioreagents, E-F, Hematology and Histology, Research Essentials,
Quality Level   200
form   powder
composition   Dye content, ≥75%
application(s)   hematology: suitable
  histology: suitable
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI   1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
Gene Information   human ... PTPN1(5770), PTPRF(5792)



Evans blue has been used to check cell viability. It has been used to study BBB permeability.


10, 50, 100 g in glass bottle

Biochem/physiol Actions

Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should Product E2129, Evans Blue, be dissolved?
This product is soluble in water, alcohol, acids and alkalies.
What is the solution stability of Product E2129, Evans Blue?
This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.
What is the difference between Evans Blue Products E2129 and 206334?
Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.
What is the shelf life of Product E2129, Evans Blue?
This product has a retest date of 4 years.This information can be found on the Certificate of Analysis.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Evans Blue, Product E2129, be used as a counterstain for FITC?
This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).
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