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  • E2156 - Monoclonal Anti-EGF Receptor antibody produced in mouse

E2156 Sigma-Aldrich

Monoclonal Anti-EGF Receptor antibody produced in mouse

clone 225, purified from hybridoma cell culture

Synonym: Anti-EGFR, Anti-Epidermal Growth Factor Receptor



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   225, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
Featured Industry   Research Pathology
UniProt accession no.   P00533
Gene Information   human ... EGFR(1956)


General description

Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.


partially purified EGF receptors from human A-431 cells.


Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.

Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).

Physical form

0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Biochem/physiol Actions

EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.

EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

EGF Signaling: Tracing Cancer's Path

The epidermal growth factor (EGF) family is a group of structurally related proteins that regulate cell proliferation, migration and differentiation via tyrosine kinase receptors on target cells. The...
Keywords: Apoptosis, Cancer, Cell proliferation, Cloning, Gene expression, Growth factors, Ligands, Morphogenesis, Phosphorylations, Transcription, Transduction, transformation

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Peer-Reviewed Papers


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