• USA Home
  • E2156 - Monoclonal Anti-EGF Receptor antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
E2156 Sigma-Aldrich

Monoclonal Anti-EGF Receptor antibody produced in mouse

clone 225, purified from hybridoma cell culture

Synonym: Anti-EGFR, Anti-Epidermal Growth Factor Receptor

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   225, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
Featured Industry   Research Pathology
UniProt accession no.   P00533
Gene Information   human ... EGFR(1956)

Description

General description

Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.

Immunogen

partially purified EGF receptors from human A-431 cells.

Application

Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.

Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).

Physical form

0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Biochem/physiol Actions

EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.

EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

EGF Signaling: Tracing Cancer's Path

The epidermal growth factor (EGF) family is a group of structurally related proteins that regulate cell proliferation, migration and differentiation via tyrosine kinase receptors on target cells. The...
Keywords: Apoptosis, Cancer, Cell proliferation, Cloning, Gene expression, Growth factors, Ligands, Morphogenesis, Phosphorylations, Transcription, Transduction, transformation

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

HPA001200 Anti-EGFR antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
E1282 Anti-Epidermal Growth Factor Receptor antibody produced in goat, affinity isolated antibody, lyophilized powder
E1157 Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat, affinity isolated antibody, lyophilized powder
E0532 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 102618, purified immunoglobulin, lyophilized powder
E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 29.1, ascites fluid
E3641 Epidermal Growth Factor Receptor human, buffered aqueous glycerol solution, 5,000-30,000 units/mg protein (Lowry)
E3138 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone F4, ascites fluid
E2645 Epidermal Growth Factor Receptor human, lyophilized powder, ≥15,000 units/mg protein (Bradford)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?