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E2636 Sigma-Aldrich

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase Conjugates, Antibodies, Antibody Conjugates, Avidin / Biotin Reagents, Avidin-Biotin Complex Reagents,
Quality Level   200
form   buffered aqueous solution
application(s)   dot blot: 1:300,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
  indirect ELISA: 1:60,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin -Alkaline Phosphatase has been used in:
• Dot blot
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Indirect ELISA
• Enzyme linked immunosorbent assay (ELISA)
• Enzyme-linked immunosorbent spot (ELISPOT) analysis
• Competitive ELISA

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qual...
Keywords: Absorption, Adsorption, Amplification, Apoptosis, Cancer, Clinical, Cryopreservation, Degradations, Deposition, Detergents, Diagnostic, Diffusion, Diseases, Enzyme activity, Enzyme-linked immunosorbent assay, Evaporation, Filtration, Flow cytometry, Gene expression, Immobilization, Indicators, Microscopy, PAGE, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Reductions, Titrations, Western blot

Protocols

Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

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Peer-Reviewed Papers
15

References

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