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E2645 Sigma-Aldrich

Epidermal Growth Factor Receptor human

lyophilized powder, ≥15,000 units/mg protein (Bradford)

Synonym: EGFR



Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
Quality Level   300
biological source   human
form   lyophilized powder
potency   >15000.00 units/mg
specific activity   ≥15,000 units/mg protein (Bradford)
mol wt   ~170 kDa
packaging   pkg of 500UN
storage condition   avoid repeated freeze/thaw cycles
color   white
solubility   glycerol: 10%, clear to slightly hazy, colorless
UniProt accession no.   P00533
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EGFR(1956)


General description

Epidermal growth factor receptor (EGFR) belongs to a family of cell surface receptors called receptor tyrosine kinases (RTKs), and is also the most studied member of this family. It is made up of an ectodomain (ECD), which contains four subdomains called DI-DIV, a transmembrane region, and a cytoplasmic tyrosine kinase domain (TKD). Binding of the ligand induces conformational changes in the ECD, which leads to the dimerization of EGFR. This results in the activation of the TKD, and the subsequent signal transduction. This receptor has a molecular weight of ∼178kDa.

Biochem/physiol Actions

EGF exerts its actions by binding to the EGF receptor (EGFR), a 170 kDa glycoprotein having EGF-activated protein tyrosine kinase activity. Even in the absence of EGF-like ligands, EGFR can participate in cellular responses elicited by several other stimuli. High levels of EGFR are expressed in approximately one third of human epithelial tumors, and in the cancers of the bladder, breast or lung that have poor clinical prognosis, hence the interest in targeting EGFR.

Epidermal growth factor receptor (EGFR) has an essential role in normal developmental processes, and is mutated in multiple cancers. It is involved in the pathogenesis of epidermoid tumors, and thus, has extreme importance as a cancer therapeutic target. Gefitinib (GEF) and erlotinib act as EGFR inhibitors, and induce apoptosis in non-small cell lung cancer (NSCLC) cells. A truncated isoform, called EGFRvIII is linked to multiple cases of head and neck squamous cell carcinoma (HNSCC). In ∼40% of glioblastoma multiforme (GBM), this gene is amplified.

Unit Definition

One unit will catalyze the incorporation of 1 pmol of phosphate from γ-32P-ATP into poly (Glu,Tyr), 4:1 at 30 °C per min.

Physical form

Lyophilized from a solution in 50 mM HEPES, pH 7.6, 150 mM NaCl, 0.05% Triton X-100, 1 mM dithiothreitol, and 10% trehalose.

Preparation Note

Affinity purified from human carcinoma A431 cells.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
Keywords: Apoptosis, Cancer, Cell proliferation, Degradations, Diseases, Electron spin resonance, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Transcription, Transduction

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


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E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 29.1, ascites fluid
E3641 Epidermal Growth Factor Receptor human, buffered aqueous glycerol solution, 5,000-30,000 units/mg protein (Lowry)
E3138 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone F4, ascites fluid
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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