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  • E3156 - Anti-AtEB1b (C-terminal) antibody produced in rabbit

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E3156 Sigma-Aldrich

Anti-AtEB1b (C-terminal) antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   Arabidopsis sp.
application(s)   western blot: 1-2 μg/mL using leaves extract (cytosolic fraction) of transgenic Arabidopsis seedlings expressing YFP-tagged AtEB1b
clone   polyclonal
concentration   ~2 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 60 kDa (additional band at 50 kDa representing degradation products)
shipped in   dry ice
storage temp.   −20°C
Gene Information   Arabidopsis thaliana ... ATEB1A(823923), ATEB1B(836370)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 205-221 located in a region near the C-terminus of Arabidopsis AtEB1b, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants.

Application

Anti-AtEB1b (C-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using leaves extract (cytosolic fraction) of transgenic Arabidopsis seedlings expressing YFP-tagged AtEB1b.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

End-binding 1 (EB1) proteins are plus-end-tracking proteins that are specifically localized to growing microtubule plus ends that regulate microtubule and interact with intracellular targets. The plant-specific EB1 subtype functions on the spindle microtubules by accumulating in the prophase nucleus. The microtubule-end-binding protein expressed in Arabidopsis thaliana cells (is called as AtEB1a) is fused with green fluorescent protein (GFP) resulting in labelling of spindle poles. During interphase, AtEB1a-GFP labels the microtubule plus end and also marks the minus end. The microtubule associated protein END BINDING 1b (EB1b) is involved as mutants and dampens root responses to touch and enhances gravitropism. In animals, EB1 proteins have acidic C-terminal tails that might induce an auto-inhibitory conformation.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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